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Improved multiplex-PCR method for the simultaneous detection of food bacteria producing biogenic amines

机译:改进的多重PCR方法可同时检测产生生物胺的食用细菌

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摘要

The presence of biogenic amines in foods is of considerable\udpublic concern for the food industry and the regulatory\udagencies, since given the potential health hazard, there is a\udgrowing demand from consumers and control authorities to reduce\udthe allowable limits of biogenic amines in foods and beverages.\udRapid and simple methods are needed for the analysis\udof the ability to form biogenic amines by bacteria in order to\udevaluate the potential risk of bacterial occurring in some food\udproducts. Analytical chromatographic methods used for routine\udbiogenic amine analysis of food substrates have been applied\udto bacterial cultures. Specifi c differential culture media for the\udpresumptive identifi cation of biogenic amine-producer bacteria\udhave been developed. Recently, several PCR based methods targeting\udamino acid decarboxylases have been described. These\udlatter molecular methods, in addition to its rapidity and specifi city,\udoffer the advantage of the identifi cation of producer bacteria\udbefore the amine is synthesized
机译:食品中生物胺的存在是食品工业和监管部门的关注\\公众普遍关注的问题,因为考虑到潜在的健康危害,消费者和控制当局对\降低生物胺的允许限量的需求不断增加。 \ ud需要快速,简单的方法来分析/由细菌形成生物胺的能力,以便\评估某些食品中的细菌潜在危害。用于食品底物常规\生源胺分析的分析色谱方法已应用于细菌培养。已经开发了用于\生物识别产胺细菌的推测性鉴定的特定差异培养基。近来,已经描述了几种靶向\双氨基酸脱羧酶的基于PCR的方法。这些\其他分子方法,除了其快速性和适用性外,\在合成胺之前还具有识别生产菌的优势。

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